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Polyacrylamide gel electrophoresis as the most commonly used and effective separation technology, is also widely used in the separation and identification of polysaccharides and their complexes. Nowadays, the methods of polysaccharide staining mainly include PAS staining, Alcian blue staining and fluorescent staining. The principle of PAS staining is to use high iodized acid (HIO4) as an oxidant, destroy the C-C bond of polysaccharide compound structure, the polysaccharide oxidation into polymer aldehyde compounds, the aldehyde compounds generated with Schiff reagent combination, produce purple complex.
Glycoprotein Gel PAS Stain Kit is a convenient, rapid and sensitive colorimetric kit for specific staining of glycoproteins isolated by polyacrylamide gel electrophoresis (PAGE). By treating the gel containing the separated protein with an oxidant, the cis-diol glycan group in the glycoprotein is oxidized to an aldehyde group, which binds to the Schiff Reagent, resulting in a magent-colored chromatographic band.This kit can be used for staining 10-15 mini gels, and its actual detection sensitivity is related to the degree of glycosylation of the target protein.
