Seamless cloning technology based on the principle of recombination does not rely on cumbersome digestion, ligation steps, and does not require end-complementing operations. According to the recombination of DNA fragments and 15-25 nt homologous sequences at the end of linearized vectors, inserts can be cloned to any site of any vector, and the vector self-connection background is very low, which is a simple, fast and efficient DNA directional cloning technology.This product can complete the recombination of single or multiple DNA fragments in one reaction, and the recombination of a single fragment can be completed in only 5 minutes at the earliest, with a positive rate of more than 95%. The Taq enzyme used in Mix has a higher fidelity and significantly increases the success rate of seamless cloning. A transformation enhancer is also added to the mix, which greatly increases the number of transformants. While ensuring fidelity and conversion efficiency, this product further optimizes the components to significantly improve its stability and withstand heat and oxygen environments.