This product is a one-step Real-Time RT-PCR reagent. The included SYBR Green I fluorescent dye binds to all double-stranded DNA, allowing the product to be used to detect a wide range of different target sequences without synthesizing specific labeled probes. When using this product, reverse transcription and quantitative PCR are performed in the same reaction without adding reagents or openning the cap, avoiding contamination and improving experimental efficiency. Loss of RNase H activity in a new and efficient reverse transcriptase reduces RNA degradation in reverse transcription reactions. The enzyme has a high reverse transcription efficiency and can perform a good reverse transcription reaction on a small amount of RNA template. RNA templates with high affinity for RNA, high GC content, and complex secondary structure. The new high-efficiency hot-start enzyme is actively blocked at room temperature, thus avoiding non-specific amplification due to non-specific binding of primers and templates or primer dimers, improving the accuracy of real-time PCR reactions. The included buffer system maximizes the efficiency of both enzymes. This product has high sensitivity, high specificity, and a wide linear range, which makes the quantification of the target gene more accurate.