β-Glucuronidase ( β-GD ) Activity Assay Kit
Cat.No：BC6155 Solarbio
Detection Method：Spectrophotometer/Microplate Reader
Storage：Store at 2-8℃
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> Biochemical Assay Kits > Carbohydrate Metabolism Series > Glycometabolic Series > β-Glucuronidase ( β-GD ) Activity Assay Kit
Product Information
| Name | β-Glucuronidase ( β-GD ) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8℃ |
| Unit | kit |
| Specification | 100T/48S |
β-glucuronidase (β-GD, EC 3.2.1.31)，a lysosomal acid hydrolase responsible for catalyzing the depolymerization of β-D-glucuronide, has become a feasible molecular target for a variety of therapeutic applications. The enzyme is widely distributed in mammalian tissues, body fluids, and microbiota, and can also be found in plants, fish, insects, and mollusks. For humans, the enzyme can be used as a reliable biomarker for tumor diagnosis and clinical treatment evaluation due to increased expression of β-GD in necrotic areas and other body fluids in patients with different forms of cancer, such as breast cancer, cervical cancer, colon cancer, lung cancer, kidney cancer and leukemia.
β-glucuronidase can catalyze the substrate 4-nitrophenyl-β-D-glucuronide to produce p-Nitrophenol, which has a characteristic absorption peak at 405 nm. Because the absorbance value of p-nitrophenol is proportional to the content, the enzyme activity of β-glucuronidase can be calculated by measuring the content of p-nitrophenol produced per unit time.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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