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Soil Superoxide Dismutase (S-SOD) Activity Assay Kit ( WST Method )
Storage:Store at 2-8℃
Soil superoxide dismutase (S-SOD) plays an important role in soil ecosystem, which can catalyze the disproportionation of superoxide anions to produce H2O2 and O2. SOD is not only a superoxide anion scavenging enzyme, but also a major H2O2-producing enzyme, which plays an important role in the biological antioxidant system.
Superoxide anion (O2-) is produced by the reaction system of xanthine and xanthine oxidase. O2- can react with WST-1 to form water-soluble yellow formazan, which has an absorption peak at 450 nm. SOD can scavenge O2-, thereby inhibiting the formation of formazan；the deeper the yellow color of the reaction solution, the lower the activity of SOD, and the higher the activity.
Superoxide anion (O2-) is produced by the reaction system of xanthine and xanthine oxidase. O2- can react with WST-1 to form water-soluble yellow formazan, which has an absorption peak at 450 nm. SOD can scavenge O2-, thereby inhibiting the formation of formazan；the deeper the yellow color of the reaction solution, the lower the activity of SOD, and the higher the activity.
Remark：
These protocols are for reference only. Solarbio does not independently validate these
methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
