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Endoplasmic Reticulum Red Fluorescent Probe
Storage:-20℃ for 6 months, avoid light.
This product is a red fluorescent probe of endoplasmic reticulum with cell membrane permeability, which is highly selective for endoplasmic reticulum and can be used for specific fluorescent staining of endoplasmic reticulum in live cells.
This product will solidify and stick to the bottom of the centrifuge tube, the tube wall or the tube cap under the condition of 4℃ and ice bath, and can be used after being completely thawed in a water bath at 20-25℃. For small amounts of liquid, centrifuge for a few seconds before each use to allow the liquid to settle sufficiently to the bottom of the tube.
Fluorochromes have quenching problems, please try to avoid light to slow down the fluorescence quenching.
This product is suitable for fluorescent staining of endoplasmic reticulum in living cells, but not for fluorescent staining of endoplasmic reticulum in fixed cells. If the stained cells require fixation, fix with 4% paraformaldehyde for 2 min at 37 ℃.
Stained cells cannot be permeabilized with Triton X-100, which will result in loss of fluorescent staining.
This product will solidify and stick to the bottom of the centrifuge tube, the tube wall or the tube cap under the condition of 4℃ and ice bath, and can be used after being completely thawed in a water bath at 20-25℃. For small amounts of liquid, centrifuge for a few seconds before each use to allow the liquid to settle sufficiently to the bottom of the tube.
Fluorochromes have quenching problems, please try to avoid light to slow down the fluorescence quenching.
This product is suitable for fluorescent staining of endoplasmic reticulum in living cells, but not for fluorescent staining of endoplasmic reticulum in fixed cells. If the stained cells require fixation, fix with 4% paraformaldehyde for 2 min at 37 ℃.
Stained cells cannot be permeabilized with Triton X-100, which will result in loss of fluorescent staining.
Remark：
These protocols are for reference only. Solarbio does not independently validate these
methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
