Glycerol-3-Phosphate Oxidase Activity Assay Kit
Storage:Store at -20℃,6months
Glycerol-3-phosphate oxidase (GPO, EC 1.1.3.21) is an enzyme present in the cytoplasm, mainly involved in a part of the fat metabolism pathway, known as the glycerol phosphate pathway.
Glycerol phosphate oxidase (GPO) oxidizes glycerol phosphate to dihydroxyacetone phosphate and produces hydrogen peroxide. Under the action of α-glycerophosphate dehydrogenase, NADH and dihydroxyacetone phosphate produce α-glycerophosphate and NAD+, causing a decrease in absorbance at 340nm. The activity of glycerol-3-phosphate oxidase can be calculated by measuring the change of NADH.
Glycerol phosphate oxidase (GPO) oxidizes glycerol phosphate to dihydroxyacetone phosphate and produces hydrogen peroxide. Under the action of α-glycerophosphate dehydrogenase, NADH and dihydroxyacetone phosphate produce α-glycerophosphate and NAD+, causing a decrease in absorbance at 340nm. The activity of glycerol-3-phosphate oxidase can be calculated by measuring the change of NADH.
Remark:
These protocols are for reference only. Solarbio does not independently validate these
methods.
Note:
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