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My CartReactivity：Rat,Human,Mouse,Human;Mouse;Rat;Monkey,Monkey
Application：WB,IHC
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：7431
Host：Rabbit
Qty：
Size：
| Name | Anti-VIM Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Bovine;Pig |
| Dilution Ratio | WB 1:1000-3000.IHC 1:50-200. |
| Target | VIM |
| Background | This gene encodes a type III intermediate filament protein. Intermediate filaments，along with microtubules and actin microfilaments，make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm，and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment，migration，and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. |
| Swiss Prot | P08670 |
| Gene ID | 7431 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | Recombinant protein of human VIM |
| Public Immunogen Range | 1-320aa |
| Calculated MW | 54kDa |
| Observed MW | 60kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | bottle |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human;Mouse;Rat;Monkey Monkey Rat Mouse Human |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with VIM antibody diluted at 1:2000;Lane: COS7,A549,HeLa,HEK293,293T,U251.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with VIM antibody diluted at 1:100.
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