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Reactivity：Common Species
Sample Type：Serum, plasma, Cell culture supernatant
Sensitivity：0.12 ng/ml
Storage：2-8℃,6 months
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| Name | Cortisol ELISA Kit |
| Storage | 2-8℃,6 months |
| Detection Target | Cortisol |
| Sample Type | Serum, plasma, Cell culture supernatant |
| Reactivity | Common Species |
| Reaction Type | Competitive-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 0.23 ng/ml-15 ng/ml |
| Sensitivity | 0.12 ng/ml |
| Specificity | Do not react with Cortisol etc. |
| Precision | Coefficient of variation is < 10% |
| Recovery | 80%-120% |
| Assay Time | 3.5h |
| Detection Principle | Solarbio ELISA试剂盒采用基于固相竞争法的酶联免疫吸附检测技术。兔抗小鼠抗体预包被在高亲和力的酶标板上。皮质醇特异性的单克隆抗体加入至孔中，经过孵育，与固相抗体结合。洗涤后，加入辣根过氧化物酶标记的皮质醇和未标记的皮质醇或样本，竞争结合单克隆抗体上有限的结合位点。在洗涤去除未结合的物质后，加入显色底物，颜色反应的深浅与皮质醇的浓度成反比。加入终止液终止反应，在450nm波长(参考波长630nm)测定吸光度值。本试剂盒已验证人、小鼠和大鼠血清样本，但其它物种的皮质醇预计也可被检测。 |
| Unit | Box |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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