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My Cart> Analytical Standards > HPLC Kit > Derivatization Standard Kit > Amino acid derivatization kit (PITC method, without standards)
Storage：Stroe at 2-8℃，1 year.
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| Name | Amino acid derivatization kit (PITC method, without standards) |
| Storage | Stroe at 2-8℃，1 year. |
| Background | Derivatization is mainly used to improve the detectability of the target substance and to improve the response of the detector to the target substance.PITC (Phenyl Isothiocyanate) derivatization is mainly used for the analysis of amino acids, and is based on the principle that the primary amino group of an amino acid reacts with PITC under alkaline conditions. The α-amino group of the amino acid is nucleophilic, while the isothiocyanate group (-N=C=S) in phenyl isothiocyanate is an electrophilic reagent, and the two are capable of undergoing a nucleophilic addition reaction. The reaction first produces an anilino thiocarbonyl (PTC-) amino acid intermediate, which is unstable and will undergo a cyclization reaction under acidic conditions to form a phenylthioacetylurea (PTC-) amino acid. (PTC-) Amino acids have good stability and chromatographic properties, and are very suitable for separation and detection by high performance liquid chromatography (HPLC) and other analytical methods.
Translated with DeepL.com (free version) |
| Unit | kit |
| Specification | 25T 50T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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