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My CartReactivity：Human,Mouse,Rat
Application：IHC,ELISA,IF,WB
Clone Type：Monoclonal Antibody
Storage：Store at -20℃.Avoid freeze/thaw cycles.
Gene ID：8678
Purity：Beclin 1
Qty：
Size：
| Name | Anti-Beclin 1 Monoclonal Antibody |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Dilution Ratio | WB 1:1000-5000.IHC 1:250-1000.IF 1:50-500. |
| Target | Mouse |
| Background | Beclin 1 also known as ATG6 or VPS30 interacts with various cofactors (e.g.Ambra1 Barkor (Atg14) Rubicon or UVRAG) to regulate the lipid kinase Vps34 and promote the formation of the BECLIN1-Vps34-Vps15 complex hence inducing autophagy.Its function (via the BH3 domain) is inhibited by Bcl-2 or Bcl-XL.Beclin 1 (BECN1) is a crucial molecule in the control of the autophagic activity and its activity is regulated by multiple mechanisms including the post-translational modification protein-protein interaction and subcellular localization.It plays a role in crosstalk between apoptosis and autophagy.It has been reported that Beclin 1 can be cleaved into fragments of 50 37 and 35 kDa during apoptosis.It is involved in many disorders including neurodegeneration and cancer (tumorigenesis).Beclin 1 is a mammalian tumor suppressor and its gene is monoallelically deleted in 75% of ovarian 50% of breast and 40% of prostate cancers.Decreased expression of Beclin 1 has also been observed in human brain and lung tumors.The level of Beclin 1 was decreased in the affected brain regions of patients with Alzheimer’s disease early in the disease process.Recent studies have also shown that gain and loss of Beclin 1 function affects the death of heart cells. |
| Swiss Prot | Q14457 |
| Gene ID | 8678 |
| Subcellular Location | Golgi apparatus trans-Golgi network membrane Peripheral membrane protein Note=Interaction with ATG14 promotes translocation to autophagosomes Expressed in dendrites and cell bodies of cerebellar Purkinje cells |
| Immunogen | Recombinant protein of human Beclin 1 |
| Public Immunogen Range | 141-450aa |
| Calculated MW | 52kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃.Avoid freeze/thaw cycles. |
| Purity | Beclin 1 |
| Conjugation Concentration | bottle |
| Conjugation | 52-60kDa |
| Specification | 50ul 100ul |
| Reactivity | Human Mouse Rat |
| Application | WB IHC IF ELISA |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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