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My Cart> Biochemical Assay Kits > Other Biochemical Series > Other Series > β-Hydroxybutyrate Dehydrogenase（β－HBDH）Activity Assay Kit
Detection Method：Spectrophotometer/Microplate Reader
Storage：Store at -20℃，6months
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| Reagents Required But Not Provided | If you use 96-well plate determination, you need to use 96-well UV plate, recommended cat. YA0602 |
| Name | β-Hydroxybutyrate Dehydrogenase（β－HBDH）Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6months |
| Unit | kit |
| Specification | 100T/96S |
β-hydroxybutyrate dehydrogenase ( β-HBDH;EC1.1.1.30 ) is a key enzyme in the process of ketone body redox reaction. Ketone bodies are an important marker for detecting metabolic control, so accurate detection of β-hydroxybutyrate dehydrogenase activity is critical.
At pH 7.0 and 37 °C, acetoacetate is reduced to 3-hydroxybutyrate or decarboxylated to acetone by β-HBDH, and NADH is oxidized to NAD +.NADH has a characteristic absorption peak at 340 nm. The activity of β-HBDH can be calculated by detecting the change of absorbance at 340 nm.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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