{{cart_num}}
My Cart> Biochemical Assay Kits > Carbohydrate Metabolism Series > Glycometabolic Series > Glucosinolate Content Assay Kit
Detection Method：Spectrophotometer
Storage：Store at -20℃，6months
Qty：
Size：
| Name | Glucosinolate Content Assay Kit |
| Detection Method | Spectrophotometer |
| Storage | Store at -20℃，6months |
| Unit | kit |
| Specification | 50T/24S |
Glucosinolates, also known as glucosinolates (GS), also known as (Z)-cis-N-hydroximinosulfateesters, are a class of plant secondary metabolites containing nitrogen and sulfur. Glucosinolates and myrosinase together constitute a special substrate-enzyme system in plants, namely glucosinolate-myrosinase system. This system is considered to be an important defense system in cruciferous plants. It plays an important role in plant defense against pathogen infection and animal and insect biting.
Glucosinolates can be catalyzed by myrosinase to produce glucose. Glucose is catalyzed by hexokinase ( HK ) to produce glucose-6-phosphate ( G-6-P ), and G-6-P is catalyzed by D-glucose-6-phosphate dehydrogenase ( G6PDH ) to produce glucose phosphate ( D-Gluconate-phosphate ), accompanied by NADP reduction to NADPH. Under the action of 1-mPMS, WST-1 can react with NADPH to produce water-soluble Formazan, which has a maximum absorption peak at 450 nm, according to which the content of glucosinolates can be calculated.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Sorry, there is no experimental images.
Sorry, there is no more information.
Sorry, there is no more information.
