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My Cart> Biochemical Assay Kits > Carbohydrate Metabolism Series > Glycolysis Series > α-Hydroxybutyrate Dehydrogenase（α－HBDH）Activity Assay Kit
Detection Method：Spectrophotometer/Microplate Reader
Storage：Store at -20℃，6months
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| Reagents Required But Not Provided | If you use 96-well plate determination, you need to use 96-well UV plate, recommended cat. YA0602 |
| Name | α-Hydroxybutyrate Dehydrogenase（α－HBDH）Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6months |
| Unit | kit |
| Specification | 100T/96S |
α-hydroxybutyrate dehydrogenase ( α-HBDH;EC.1.1.1.27 ), which is widely present in the cytoplasm of various tissue cells in the human body, with the highest content in myocardial tissue. Its content determination can be used to evaluate myocardial infarction, hepatitis, macroglobulinemia, alcoholic liver disease, diabetic nephropathy and other diseases.
Under the catalysis of α-hydroxybutyrate dehydrogenase, α-hydroxybutyrate is produced by α-ketobutyrate and reduced coenzyme I (NADH), and NADH is oxidized to oxidized coenzyme I (NAD+). The oxidation rate of NADH is proportional to the activity of α-HBDH in the sample. By monitoring the rate of decrease in absorbance at 340 nm, the activity of α-HBDH in the sample can be calculated.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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