Sperm BWW Culture Medium

This product is suitable for the separation of cells, subcellular components and large viruses(>70S), uniform osmotic pressure, low viscosity, adjustable to physiological ionic strength and pH, it has mild separation conditions, non-toxic to cells, and it can be sterilized.

Percoll separation solution is a kind of silica gel particles treated with polyvinylpyrrolidone, which can form a continuous density gradient after high-speed centrifugation. Because of the low diffusion constant of Percoll, the gradient is very stable. When the pre-formed density gradient was used in Percoll separation solution, satisfactory cell separation results could be achieved at low centrifugal force (200~ 1000 g) in ten minutes. In addition, Percoll does not penetrate the biofilm and is not toxic to cells, so it is widely used to isolate cells, subcellular components, bacteria and viruses, and to separate damaged cells and their fragments from intact living cells. Percoll is often used to isolate and communicate plant protoplasts, nuclei, chloroplasts, and mitochondria.

The advantages of Percoll separation solution:

Percoll is an ideal medium at present because of its low permeability, non-penetration, high density and non-toxicity. Percoll density gradient centrifugation has been successfully used to separate animal cells and their organelles for many times. More than ten kinds of animal cells including human blood cells, bone marrow cells, red blood cells, white blood cells, lymphocytes, hepatocytes and so on have been purified.

Note:

1.Percoll cell separation solution should avoid keeping it in strong light during storage and incubation.

2.The cap of the reagent bottle should be closed tightly to prevent evaporation and pollution.

The number of Percoll cell separation solution produced by Solarbio is P8370, it is suitable for the isolation of cells, subcellular components and large viruses (>70S), uniform osmotic pressure, low viscosity, adjustable to physiological ionic strength and pH, it has mild separation conditions, non-toxic to cells, and it can be sterilized. The density is 1.13 g/mL.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.