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DAPI Solution(ready to use)
CAS:28718-90-3
Storage: -20℃, avoid light, valid for 1 year.
Purity:≥90% (HPLC和 TLC)
Product Information
| CAS | 28718-90-3 |
| English Name | DAPI Solution(ready-to-use) |
| Synonym | DAPI |
| Purity | ≥90% (HPLC和 TLC) |
| Unit | Bottle |
| Molecular Formula | C16H15N5·2HCl |
| Molecular Weight | 350.25 |
| Storage | -20℃, avoid light, valid for 1 year. |
| Specification | 10ml 50ml |
DAPI(4 ', 6-diamidino-2-phenylindole dihydrochloride) is A fluorescent dye that can bind most of the A and T bases in DNA and is often used for fluorescence microscopy. Because DAPI can penetrate intact cell membranes, it can be used for staining of both live and fixed cells. When DAPI is bound to double-stranded DNA, the maximum absorption wavelength is 358nm and the maximum emission wavelength is 461nm. The emission light of DAPI is blue, and the emission wavelength of DAPI and green fluorescent protein GFP or Texas Red dye (red fluorescent dye) only partially overlaps, which can be used for multiple fluorescent staining on a single sample.
This product is DAPI aqueous solution with a purity of ≥90%. It is a ready-use working solution and can be directly used for nuclear staining of fixed cells or tissues.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：
These protocols are for reference only. Solarbio does not independently validate these
methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Technical Q&A
Q&A（Total 8
Users）
2023-08-10 11:44:11
2023-06-14 11:27:48
该溶液一般染色多长时间2023-05-30 17:44:43
Administrator您好，染色时间可参考说明书，建议染色时间根据自己实际染色情况调整。
对于组织样品必须得固定才能染色吗2023-05-17 17:44:53
Administrator您好，DAPI透膜能力相对不强，需要组织固定后染色
2023-05-09 14:14:07