人慢性髓系白血病细胞；K562
Cat.No：SCC-121813 Solarbio
Storage：Store at -70℃，avoid freeze/thaw cycle.
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Endocrinology & Hormones
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Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
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Spectrophotometer
Centrifuge
Shaker
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Product Information
| Name | K562 |
| Storage | Store at -70℃，avoid freeze/thaw cycle. |
| Transport Condition | The product is shipped on dry ice pack. |
| Culture Condition | IMDM＋10% FBS＋1% P/S |
| Cell Type | Suspension cell |
| Background | Continuous cell line K-562 was established by Lozzio from the pleural fluid of a dying 53-year-old female patient with chronic myeloid leukemia.K-562 cells are classified into highly undifferentiated granulocyte group.Erson et al.'s study on the surface membrane properties of K-562 cells showed that K-562 cells were a human erythroleukemia cell.K-562 cells are widely used as highly sensitive receptors in vitro in natural kill assays, and See Pross et al.used K-562 cells for detailed in vitro detection of NK cells, including mathematical quantification of NK cell activity.K-562 blasts are pluripotent hematopoietic malignant cells, which can spontaneously differentiate into recognizable red blood cell progenitors, granulocyte, monocyte, etc.K-562 cells were EBNA negative. |
| Source | Bone marrow; Chronic Myelogenous leukemia (CML) |
| Note | The cells are suspension cells, please pay attention to centrifuge to collect cell suspension; Do not throw away the cell culture solution directly. |
| Unit | Piece |
| Specification | 1EA |
贴壁细胞培养
细胞名称：人慢性髓系白血病细胞；K562
形态特性：淋巴母细胞样
生长特性：悬浮生长
培养条件： RPMI 1640 (w/o Hepes) + 10%FBS
传代方法： 维持细胞浓度在1×105~2×106 cells/ml；2~3天换液1次
冻存条件：基础培养基+8%DMSO+20%FBS
特征特性： 该细胞是由Lozzio从一名53岁的慢性髓细胞性白血病急变期的女性患者的胸水中分离建立的。该细胞曾被认为来源于粒系，处于高度未分化阶段；Anderson等人作了细胞膜特性的研究后，认为该细胞是红白血病细胞系。该细胞是对自然杀伤细胞高度敏感的体外靶标，故而被广泛应用于这方面的研究。K562的原始细胞是一种具有多向分化潜能的造血系统的恶性肿瘤细胞，能自发分化为红系、粒系和单核系的可辨识的祖细胞。该细胞表达CD7（25％）。
细胞处理方法：
1. 细胞在培养瓶中培养至状态良好后灌满培养基运输，获得细胞后用酒精棉球擦拭瓶口消毒，然后在超净台中操作。
2. 将细胞转移至50mL的无菌离心管中，1000rpm离心5-10min，用完全培养基重悬细胞，适宜的密度分瓶培养。
特别注意：（如使用公共实验室或初次接触细胞培养，建议添加双抗培养）
1. 我们使用自产培养基及进口血清培养细胞，在您拿回细胞后，如想更换其它品牌培养基，请依照逐次替换的原则，先保留培养瓶中的培养基，多日多次代逐步更换，以减轻对细胞的刺激。
2. 如签收时出现培养瓶壁破裂，漏液等情况请及时拍照并联系售后。
3. 细胞任何售后问题，均需拍照存档并在2周之内及时联系客服。
相关产品及货号：
RPMI 1640 (31800 )
细胞冻存液（24800）
1×PBS缓冲液(pH7.2- 7.4)（P1020）
0.25%胰蛋白酶-EDTA消化液（T1300）
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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