293T 人胎肾细胞系
Cat.No:SCC-120511 Solarbio
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贴壁细胞培养
细胞名称:293T,人胎肾细胞
生长特性:贴壁生长
培养条件: DMEM-H: Dulbecco's Modified Eagle's Medium (DME H-21 4.5g/Liter Glucose) 10%FBS
传代方法:1:3~1:4传代,一周1~2次
冻存条件:细胞冻存液
细胞处理方法:
1. 细胞在培养瓶中培养至状态良好后灌满培养基运输,获得细胞后用酒精棉球擦拭瓶口消毒,然后在超净台中操作。
2. 如细胞生长至70%-80%,将瓶中的培养基移入无菌瓶中留作培养使用,保留5-8mL培养基在37℃、5%的CO2的温箱中继续培养。细胞培养至90%-100%后,按要求消化传代。
3. 弃去培养基,用无菌PBS或者其他缓冲液清洗细胞2次,加入适量胰蛋白酶消化(EDTA胰酶),待细胞完全脱壁且细胞连接松散时再吹打,将细胞分离成单个后,加培养基混匀,分瓶培养。
特别注意:(如使用公共实验室或初次接触细胞培养,建议添加双抗培养)
1. 我们使用自产培养基及进口血清培养细胞,在您拿回细胞后,如想更换其它品牌培养基,请依照逐次替换的原则,先保留培养瓶中的培养基,多日多次代逐步更换,以减轻对细胞的刺激。
2. 如签收时出现培养瓶壁破裂,漏液等情况请及时拍照并联系售后。
3. 细胞任何售后问题,均需拍照存档并在2周之内及时联系客服。
相关产品及货号:
高糖DMEM (12100)
细胞冻存液(24800)
1×PBS缓冲液(pH7.2- 7.4)(P1020)
0.25%胰蛋白酶-EDTA消化液(T1300)
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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