Alcian Blue Stain Kit，pH 2.5
Cat.No：G1560 Solarbio
Storage：Store at RT，avoid light，6 months
Molecular Formula：C56H68N16S4Cl4Cu
CAS：75881-23-1
Qty：
-
+
Size：
{{cart_num}}
My CartTel: +86 010 50973105
Products
Secondary Antibodies
SAlexa Fluor-Conjugated
Other Fluorescent-Conjugated
Other-Conjugated
Colloidal Gold Reagent
Alkaline Phosphatase(AP)-Conjugated
Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
Phycoglobin(PE)-Conjugated
RBTIC-Conjugated
Peroxidase (HRP)-Conjugated
Secondary Antibodies
SAlexa Fluor-Conjugated
Other Fluorescent-Conjugated
Other-Conjugated
Colloidal Gold Reagent
Alkaline Phosphatase(AP)-Conjugated
Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
Phycoglobin(PE)-Conjugated
RBTIC-Conjugated
Peroxidase (HRP)-Conjugated
Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Product Information
| Name | Alcian Blue Stain Kit，pH 2.5 |
| CAS | 75881-23-1 |
| Molecular Formula | C56H68N16S4Cl4Cu |
| Storage | Store at RT，avoid light，6 months |
| Unit | Kit |
| Specification | 3*50ml 3*100ml |
Product Introduction:
Alcian, also known as alcian blue 8GX, is a conjugated dye of copper titanium cyanine, which was originally used for textile fiber dyeing. This cationic dye combines with acidic groups, that is, alcian blue forms an insoluble complex with anionic groups such as carboxyl groups and sulfate groups contained in the tissue. Alcian blue is formed by connecting the central copper containing phthalocyanine ring with four isothiourea groups through thioether bonds. It can be combined with different anion groups at different pH and colored. Ordinary acidic mesenchymal mucin can be stained at pH 2.5, sulfated mucin can be stained at pH 1.0 and complex acid connective tissue mucin can be stained at pH 0.5, and neutral mucin is not stained.
Alcian Blue Stain Kit, pH 2.5 can stain glycoprotein modified by sialic acid, also known as hyaluronic acid. It can be used for cartilage staining, mucin staining and determination of intestinal metaplasia. It can also be used for identification of mucinous epithelial tumors and proof of whether mucinous substances are contained in tumors. This kit contains nuclear fast red double staining solution, which can be used for coloring and morphological observation of nucleus.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
小鼠小肠石蜡切片
酸性粘液质着蓝色，细胞核和嗜碱性物质着粉红色
Related Products
Related Paper
Adverse effect of oxidized cholesterol exposure on colitis is mediated by modulation of gut microbiota
Click to check >>Author：Chi Yan, Shou-He Huang, Hua-Fang Ding, Erika Kwek, Jian-Hui Liu, Zi-Xing Chen, Ka Ying Ma, Zhen-Yu Chen
IF：13.6000
Publish_to：JOURNAL OF HAZARDOUS MATERIALS
PMID：37467611
Viscoelasticity microenvironment constructed by self-crosslinking hyaluronan hybrid hydrogels regulates chondrogenic differentiation of mesenchymal stem cells
Click to check >>Author：Hongfu Cao, Zhulian Li, Yafang Chen, Jiayi Zhu, Manyu Chen, Haoyuan Lei, Yun Xiao, Jie Liang, Tun Yuan, Yong Sun, Yujiang Fan, Xingdong Zhang
IF：13.1000
Publish_to：COMPOSITES PART B-ENGINEERING
PMID：
Multifunctional mesoporous polydopamine near-infrared photothermal controlled release of kartogenin for cartilage repair
Click to check >>Author：Zhen-Yu Luo, Zun-Han Liu, Hao-Da Yu, An-Jing Chen, Ze Du, Yong-Rui Cai, Xiao-Xue Fu, Shu-E Jin, Jia-Li Chen, Zong-Ke Zhou, Wei-Nan Zeng
IF：9.4170
Publish_to：MATERIALS & DESIGN
PMID：
CD73 mediated host purinergic metabolism in intestine contributes to the therapeutic efficacy of a novel mesenchymal-like endometrial regenerative cells against experimental colitis
Click to check >>Author：Bo Shao, Shao-hua Ren, Zhao-bo Wang, Hong-da Wang, Jing-yi Zhang, Hong Qin, Yang-lin Zhu, Cheng-lu Sun, Yi-ni Xu, Xiang Li, Hao Wang
IF：8.7860
Publish_to：Frontiers in Immunology
PMID：37180168
The Effect of miR-140-5p with HDAC4 towards Growth and Differentiation Signaling of Chondrocytes in Thiram-Induced Tibial Dyschondroplasia
Click to check >>Author：Hu Shilin, Chen Bo, Zhou Jiannan, Liu Fangqi, Mao Tianjiao, Pathak Janak L., Watanabe Nobumoto, Li Jiang
IF：7.4000
Publish_to：Journal of Translational Medicine
PMID：37268950
Dynamic changes of enhancer and super enhancer landscape in degenerated nucleus pulposus cells
Click to check >>Author：Guowang Li, Yuxiang Kang, Xiangling Feng, Guohua Wang, Yue Yuan, Zhenhua Li, Lilong Du, Baoshan Xu
IF：5.7810
Publish_to：Life Science Alliance
PMID：37012048
Hydrogen gas ameliorates acute alcoholic liver injury via anti-inflammatory and antioxidant effects and regulation of intestinal microbiota
Click to check >>Author：Haixia Liu, Xing Kang, Peng Ren, Xiaoyu Kuang, Xiaodan Yang, Hao Yang, Xiaorong Shen, Huan Yan, Yongbo Kang, Fan Zhang, Xiaohui Wang, Linzhi Guo, Weiping Fan
IF：5.7140
Publish_to：INTERNATIONAL IMMUNOPHARMACOLOGY
PMID：37196556
Biogenic selenium nanoparticles synthesized by Lactobacillus casei ATCC 393 alleviate diquat-induced intestinal barrier dysfunction in C57BL/6 mice through their antioxidant activity
Click to check >>Author：乔磊
IF：4.1710
Publish_to：
PMID：32243488
Blunting ROS/TRPML1 pathway protects AFB1-induced porcine intestinal epithelial cells apoptosis by restoring impaired autophagic flux
Click to check >>Author：Wei Wang, Jing-Wen Zhang, Yu-Jie Qin, Hong-Yan Li, Yu-Xiang Dai, Hong Li
IF：3.0000
Publish_to：Translational Vision Science & Technology
PMID：37342053
Single-cell infrared phenomics identifies cell heterogeneity of individual pancreatic islets in mouse model
Click to check >>Author：Zhu Chao, Zhou Qing, Wang Zuqiang, Zhang Junyou, Xu Cheng, Ruan Dike
IF：2.8000
Publish_to：MOLECULAR BIOLOGY REPORTS
PMID：37310547
Technical Q&A
Service for Scientific Research, Responsible for the Life Science
Contact Us
Address: No 85, Liandong U Valley, Middle Zone, 101102, Tongzhou Dist,
Beijing, China
Tel: +86 010 50973105
Email: info@solarbio.com
Order Guide
Service Guarantee
Resources Download
Copyright © 2005-2021 Solarbio all rights reserved.
如何对孔板中的细胞进行染色，选择ph2.5还是1.0 2022-08-11 13:04:28
Administrator您好，细胞染色请参考G1565说明书操作步骤，不同pH条件下电离着色基团是有区别的，pH值为2.5时组织内的羧基电离，带有一个负电荷，与阿利新蓝中的阳离子形成盐键，是带有羧基的组织（如蛋白多糖/透明质酸以及上皮酸性黏蛋白）染色。pH值为1.0时，组织内的硫酸根电离，带有一个负电荷，与阿利新蓝中的阳离子形成盐键，使带有硫酸根的组织（如硫酸黏液物质）染色。中性黏蛋白(如胃黏膜和Brunner腺体部位的中性黏蛋白）不能与阿利新蓝反应。根据您想鉴别区分的阳性物质选择用哪一种pH值的阿利新蓝染色液。