Product Introduction:
Conventional Tris-SDS-PAGE electrophoresis can only distinguish large molecular proteins, and the resolution of relatively small molecular weights, especially proteins below 10 kD, is extremely low. Tricine-SDS-PAGE can separate proteins and peptides with molecular weight in the range of 1-10 kD, which has become the main method of electrophoresis denaturation and separation of peptides. This product includes a complete set of reagents required for the preparation of Tricine-SDS-PAGE gel. Only distilled water is needed to prepare high-quality gel of various concentrations, which is convenient and fast. After electrophoresis, it can be directly used for test dyeing, silver dyeing, Western hybridization and other experiments.
Note:
1. 10%APS is distributed and stored at -20 degrees after preparation. APS solution is unstable, should minimize the room temperature storage time, immediately after each use back to the refrigerator to prevent failure; If it is found that the gel polymerization time is prolonged, it should be replaced with 10%APS stored at -20 degrees.
2, in the gel preparation process, especially the liquid mixing step, should try to avoid the generation of bubbles.
3. Be careful when adding distilled water to the top of the separating glue, and the speed of adding water can not be too fast.
4. Acrylamide is neurotoxic. Please wear lab clothes and disposable gloves during operation.
5, this product is only used for scientific research, can not be used for human experiments or human treatment.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
10%APS是什么?
2023-03-10 18:03:41
Administrator胶凝固剂
为什么用1.7-40kDa的marker看不到最下面两条带?
2023-03-10 17:03:35
Administrator您可以把详细情况发送到技术邮箱: service@solarbio.com
你好,这个试剂盒带的PAGE胶促凝剂有单独产品吗?2022-10-11 11:15:27
Administrator货号是T8090的PAGE胶促凝剂,P1200以及P1320凝胶制备试剂盒中的胶促凝剂单独的都是这个
用一般的通用电泳液,能够跑吗?2022-10-09 15:47:06
Administrator您好,需要用配套的阴极和阳极电泳缓冲液来跑电泳
制胶板用1.5mm的还是1.0mm的?2022-08-10 15:02:39
Administrator您好,如果两种都有建议使用1.0mm的,电泳的效果会更好一些