l analyze small aliquots from RNA preparations before Northern blotting, start-site mapping or cDNA preparation.
l Visualize the migration behavior of 5S rRNA species after high-resolution denaturing gradient electrophoresis (DGGE).
l Stain DNA in single-strand conformation polymorphism (SSCP).
l Stain DNA before amplification by PCR
Materials Contents
SYBR Green II stain is provided as a 10,000X concentrate in DMSO in the following sizes:
l 50μL (SY1040-50)
l 500 μL (SY1040-500)
Storage
Upon receipt, store the dye frozen at -20℃, protected from light in a desiccator. When stored properly, the SYBR Green II stain stock solution in DMSO is stable for six months to one year.
Note: SYBR Green II is primarily used for RNA and single-strand DNA electrophoresis. It is an alternative to EB, non-carcinogenic, super sensitive. When used for conventional electrophoresis, commercial nucleic acid MARKER must be diluted by a certain multiple (1/5-1/10), so as to obtain more ideal results.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
您好 SYBR green II的检测灵敏度是多少呢2022-08-01 15:52:54
Administrator您好,具体的使用方法和灵敏度请参考说明书或者咨询Solarbio 技术支持