Deoxyribonucleic Acid Sodium Salt from Salmon Testes
Cat.No：D8030 Solarbio
CAS：438545-06-3
Appearance：White linear
Storage：Store at 2-8℃，4 years
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> Biochemical Reagents > Nucleic Acids & Nucleotides & Derivatives > Deoxyribonucleic Acid Sodium Salt from Salmon Testes
Product Information
| Name | Deoxyribonucleic acid sodium salt from salmon testes |
| CAS | 438545-06-3 |
| Storage | Store at 2-8℃，4 years |
| Appearance | White linear |
| Source | from salmon testes |
| Unit | Piece |
| Specification | 100mg |
Product introduction
for biochemical studies.
Preparation method :
1. Weigh 2g of salmon sperm DNA in a 500ml beaker and add about 400ml of TE Buffer.
2. After dissolving, 8ml of 5 M NaCl was added to a final concentration of 0.1 M by stirring with a magnetic stirrator for 2 to 4 hours at room temperature.
3. DNA was cut by rapid aspiration of the above solution about 20 times using a 17-gauge hypodermic needle.
4. Extract once with phenol/chloroform and once with chloroform.
5. Ethanol precipitation was performed by adding 2 times the volume of precooled ethanol.
6. The DNA was recovered by centrifugation and dissolved in 100 ml of deionized water, and the OD260 value of the solution was determined.
7. After calculating the DNA concentration of the solution, the DNA solution was diluted to 10 mg/ml.
8. After boiling for 10 minutes, divide into small portions (1 ml/ portion). The samples were stored at -20 ° C.
9. After heating in a boiling water bath for 5 minutes before use, cool quickly in an ice bath.
Physical properties
Solubility: soluble in water, reference concentration 2mg/ml
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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