Papain

Product introduction

Papain is a pure natural biological enzyme product extracted from the immature fruit of papaya plant by biological engineering technology. It belongs to the sulfhydryl-containing (-SH) peptide chain endonuclease. It has protease and esterase activities, a wide range of specificity, strong enzymatic hydrolysis ability for animal and plant proteins, peptides, esters, amides, etc., and also has the ability to synthesize. "The ability to resynthesize proteinlike substances from protein hydrolysates can be used to improve the nutritional value or functional properties of animal and plant proteins."

Product features:

1. Adopting advanced bioengineering technology, membrane separation technology and vacuum freeze-drying technology, and successfully developing proprietary enzyme protection technology, reducing the loss of enzyme activity in the process, the level of advanced.

2, the United States pharmacopeia standard and China's current standard organization production, in accordance with the international quality certification system requirements, establish a strict quality control system, strict control of microorganisms in the product, to meet the national export food hygiene standards.

Product characteristics: light yellow or white solid powder.

Usage method

The reaction temperature ranged from 50 ℃ to 55℃, and the optimal temperature varied with the types and concentrations of substrates. The optimal PH value is 6-7, which can be changed by the type and concentration of substrates. The amount of addition is generally 0.1-0.3% (1000-3000U /g protein), and the reaction time is shortened or prolonged accordingly according to different substrates. The recommended amount of tender meat is 1kg of meat with 2-3g enzyme added.

The optimum amount of enzyme added must be determined according to the specific production situation.

Unit definition

Ultraviolet spectrophotometry: under the measurement conditions (37±0.2℃; PH 7.0). When the absorbance of TCA-soluble substance released from casein hydrolysis per minute at 275nm is equivalent to that of 1 microgram of tyrosine, the amount of enzyme required is one unit of activity, expressed as u/g.

Matters needing attention

Storage:

It is recommended to store in a cool and dry environment away from light. Storage temperature: below zero. Too long storage or unfavorable storage conditions will cause the enzyme activity to decrease to varying degrees. If the temperature and humidity are too high, it is necessary to increase the amount of use when used.

Safety:

Papain belongs to the pure natural enzyme preparation, is a protein, edible enzyme preparation of food like eating food containing protein, good for the human body. For some sensitive people, direct ingestion of highly concentrated enzyme powder or aerosol droplets may cause allergy, and prolonged exposure may irritate skin, eyes and mucosal tissues. It is recommended to wear a mask, eye mask and other protective equipment during the operation. The remaining or spilled enzyme powder should be treated in time. Papainase is a bioactive substance, which is easily inhibited and damaged by heavy metal ions (Fe3+, Cu2+, Hg+, Pb+, etc.) and oxidants. contact with papainase should be avoided during storage or use.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.