Products
Trypsin

CAS:9002-07-07

Storage:Store at 2-8℃

Trypsin
Cat.No:
T8151
Brand:
Solarbio

Product introduction

This product is a mixture of enzymes extracted and refined from the pancreas of pig, cow or sheep. It mainly contains protease, pancreatic amylase and pancreatic lipase. This product is widely used in proteolysis, peptide and amino acid production.

Usage method

Usually in the decomposition of proteins, the temperature is controlled at 45-55 ° C, the initial pH is adjusted to about 8.0, and the pH between 7.5 and 8.5 should be adjusted frequently as the hydrolysis proceeds (because the decomposition of proteins will produce amino acids, which will decrease the pH and affect the enzyme activity) until the end of the hydrolysis.

Unit definition

Ultraviolet spectrophotometry: under the measurement conditions (40±0.2℃; PH 8.0). When the absorbance of TCA-soluble substance released from casein hydrolysis per minute at 275nm is equivalent to that of 1μmol tyrosine, the amount of enzyme required is one unit of activity, which is expressed as u/g.

Optimum pH

8

pH Stability

7.5-8.5

Optimum temperature

45℃

Thermal stability

40~50 ℃

Matters needing attention

1. This product is only used in the field of scientific research, not edible or used in food production.

2. Inhalation of enzyme dust and enzyme fog should be avoided. If accidentally splashed on hands and eyes, should immediately rinse with water for more than 15 minutes.

Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark:
These protocols are for reference only. Solarbio does not independently validate these methods.

Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
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