T4 DNA ligase
Cat.No：T1410 Solarbio
Storage：Store at -20℃
Qty：
-
+
Size：
{{cart_num}}
My CartTel: +86 010 50973105
Products
Secondary Antibodies
SAlexa Fluor-Conjugated
Other Fluorescent-Conjugated
Other-Conjugated
Colloidal Gold Reagent
Alkaline Phosphatase(AP)-Conjugated
Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
Phycoglobin(PE)-Conjugated
RBTIC-Conjugated
Peroxidase (HRP)-Conjugated
Secondary Antibodies
SAlexa Fluor-Conjugated
Other Fluorescent-Conjugated
Other-Conjugated
Colloidal Gold Reagent
Alkaline Phosphatase(AP)-Conjugated
Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
Phycoglobin(PE)-Conjugated
RBTIC-Conjugated
Peroxidase (HRP)-Conjugated
Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Your Position: Home
> Molecular Biology > Molecular Cloning-Related Enzymes > PCR-Associated Enzyme > T4 DNA ligase
Product Information
| Name | #REF! |
| Storage | Store at -20℃ |
| Unit | Bottle |
| Specification | 60U |
Product introduction
The enzyme catalyzes the formation of phosphodiester bonds from adjacent 5 '-phosphate and 3' -hydroxyl ends of double-stranded DNA or RNA. This enzyme is not only capable of catalyzing the joining between the aligned or cohesive ends, but also of repairing single-strand cleavage in double-stranded DNA, RNA, or DNA/RNA hybrid duplexes.
Unit definition
One unit is the amount of enzyme required to ligation 50% of the HindⅢ digested λDNA fragment [5 'end concentration 0.12 μM (300 μg/ml)] in 20 μl 1X T4 DNA ligase reaction buffer at 16 ° C for 30 min.
Matters needing attention
Unlike E. coli DNA ligase, which requires NAD+ as a cofactor, the cofactor of T4 DNA ligase is ATP.
Diluted T4 DNA ligase should be stored at ? 20 ° C in 50% glycerol storage buffer (dilution buffer A, NEB #B8001). If used immediately after dilution, it can also be diluted in 1X T4 DNA ligase reaction buffer.
As long as 1 mM ATP was supplemented to the reaction system, the ligation reaction could be performed either in the four restriction enzyme buffers provided by NEB or in
T4 DNA polynucleotide kinase reaction was performed in buffer.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
Sorry, there is no experimental images.
Related Products
Sorry, there is no more information.
Related Paper
Integrated multifunctional nanoplatform for fluorescence detection and inactivation of Staphylococcus aureus
Click to check >>Author：Jing-bo Jiao, Qing Kang, Jiang-li Cao, Shuai-qi Zhang, Chen-jing Ma, Tong Lin, Ze-hui Xiao, Chu-min Zhao, Ting Du, Xin-jun Du, Shuo Wang
IF：8.8000
Publish_to：FOOD CHEMISTRY
PMID：37413833
Production of Sitobion avenae-resistant Triticum aestivum cvs using laccase as RNAi target and its systemic movement in wheat post dsRNA spray
Click to check >>Author：Asma Rafique, Amber Afroz, Nadia Zeeshan, Umer Rashid, Muhammad Azmat Ullah Khan, Muhammad Irfan, Waheed Chatha, Muhammad Ramzan Khan, Nazia Rehman
IF：3.7520
Publish_to：PLoS One
PMID：37163535
Technical Q&A
Q&A
（Total 0
User Q&A）
- There is not question and answer now, come and send an inquiry please.
My question
Service for Scientific Research, Responsible for the Life Science
Contact Us
Address: No 85, Liandong U Valley, Middle Zone, 101102, Tongzhou Dist,
Beijing, China
Tel: +86 010 50973105
Email: info@solarbio.com
Order Guide
Service Guarantee
Resources Download
Copyright © 2005-2021 Solarbio all rights reserved.