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Product introduction
The enzyme catalyzes the formation of phosphodiester bonds from adjacent 5 '-phosphate and 3' -hydroxyl ends of double-stranded DNA or RNA. This enzyme is not only capable of catalyzing the joining between the aligned or cohesive ends, but also of repairing single-strand cleavage in double-stranded DNA, RNA, or DNA/RNA hybrid duplexes.
Unit definition
One unit is the amount of enzyme required to ligation 50% of the HindⅢ digested λDNA fragment [5 'end concentration 0.12 μM (300 μg/ml)] in 20 μl 1X T4 DNA ligase reaction buffer at 16 ° C for 30 min.
Matters needing attention
Unlike E. coli DNA ligase, which requires NAD+ as a cofactor, the cofactor of T4 DNA ligase is ATP.
Diluted T4 DNA ligase should be stored at ? 20 ° C in 50% glycerol storage buffer (dilution buffer A, NEB #B8001). If used immediately after dilution, it can also be diluted in 1X T4 DNA ligase reaction buffer.
As long as 1 mM ATP was supplemented to the reaction system, the ligation reaction could be performed either in the four restriction enzyme buffers provided by NEB or in
T4 DNA polynucleotide kinase reaction was performed in buffer.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
