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Product Introduction：
DiD, DiO, DiI, DiR and DiS dyes are a family of lipophilic fluorescent dyes that can be used to stain cell membranes and other lipid-soluble biological structures. When bound to the cell membrane, the fluorescence intensity of these dyes is greatly enhanced, and they have a high quenching constant and excited state lifetime. Once the cell is stained, such dyes diffuse throughout the cell membrane and at optimal concentrations stain the entire cell membrane.
Their fluorescent colors are clearly distinguished: DiI(orange fluorescence), DiO(green fluorescence), DiD(red fluorescence) and DiR (dark red fluorescence) which allows them to be used for multicolor imaging and flow analysis of living cells. DiI and DiO can be used with standard FITC and TRITC filters, respectively. DiD can be excited by 633 nm He-Ne laser, which has longer excitation wavelength and emission wavelength than DiI, and is more valuable in cell and tissue staining. The infrared fluorescence of DiR can penetrate cells and tissues and is used for tracing in vivo imaging.
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