Ginsenoside Re

Examples of using this product（for reference only）

UPLC-PDA

The levels of the main active ingredients of MSJZT were detected using a UPLC-PDA method. Chromatographic separation was performed on a UPLC system with a photodiode array detector (PDA) and C18 (100 mm×2.1 mm, 1.7μm ) column. The column temperature was set at 30℃. The mobile phase was composed of 0.1% phosphoric acid (A) and acetonitrile (B). The optimum adsorption and elution conditions were set as follows: 0–1.5 min, 95% B; 1.5–6 min, 95–80% B; 7–10 min, 80–60% B; 10–10.5 min, 50–35% B; 10.5–12 min, 35–30% B; 12–12.5 min, 30–20% B; 12.5–16 min, 20–10% B; 16–16.5 min, 10–0% B; and 16.5–18 min, 0–95% B.

来源文献：Nie S, Zhao Y, Qiu X, Wang W, Yao Y, Yi M, Wang D. Metabolomic Study on Nude Mice Models of Gastric Cancer Treated with Modified Si Jun Zi Tang via HILIC UHPLC-Q-TOF/MS Analysis. Evid Based Complement Alternat Med. 2019 Jun 23;2019:3817879. doi: 10.1155/2019/3817879. PMID: 31341492; PMCID: PMC6612382.

UPLC

The experimental conditions were as follows: 1) Column: Waters Acquity BEH C18 column (100 mmX2.1 mm, 1.7 mm); 2) chromatographic conditions were: flow rate 0.3 mL/ min; detection wavelength (k) ? 203 nm; sample volume 5 mL; column temperature 30 ℃. With acetonitrile (B) and 0.1% phosphoric acid solution (D) as mobile phase, gradient elution: 0–3 min, 19% B; 3–5 min, 20% B; 5–12 min, 21% B; 12–13 min, 29% B; 13–25 min, 31% B; 25–32 min, 35% B; 32–35 min, 19% B; 35–41 min, 19% B

来源文献：Wu SH, Li HB, Li GL, Qi YJ, Zhang J, Wang BY. Panax ginseng root, not leaf, can enhance thermogenic capacity and mitochondrial function in mice. Pharm Biol. 2020 Dec;58(1):374-384. doi: 10.1080/13880209.2020.1756348. PMID: 32366153; PMCID: PMC7241452.

UPLC

Concentration analysis of ginsenoside was conducted on ACQUITY UPLC system (Waters, Milford, MA) equipped with diode array detector (190 to 800 nm) and a reversed-phase C18 column (2.1 × 50 mm; inner diameter 1.7 μm; Acquity UPLC BEH; Waters). The mobile phase consisted of ACN (A) and 0.00005‰ (v/v) phosphoric acid (B) with the following separation program based on Zhang et al (2018): 0 to 3 min, 17 to 19% A; 3 to 4 min, 19 to 21% A; 4 to 4.5 min, 21 to 24% A; 4.5 to 5 min, 24 to 28% A; 5 to 6.5 min, 28% A; 6.5 to 7.5 min, 28 to 30% A; 7.5 to 9.5 min, 30 to 36% A; 9.5 to 11 min, 36 to 40% A.The injection volume was 2 μL at a flow rate of 0.5 mL min?1 . The column temperature and the detection wavelength were respectively set up at 35℃ and 203 nm.

来源文献：[1] Lei X , Wang Q , Yang H ,et al.Vitrification and proteomic analysis of embryogenic callus ofPanax ginsengC. A. Meyer[J].In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association, 2021(1):57.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.