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Product Introduction：
D-Luciferin is a firefly luciferase substrate with a quantum efficiency of 0.88, 20 times higher than Luminol.It is insoluble in water,Ex (nm) 328,Em (nm) 533.
Reaction principle:
First, in the presence of magnesium ions, luciferase reacts fluorescein with ATP, and then it is oxidized to form a dioxacyclobutane structure and emits yellow-green light. Luciferin-luciferase luminescence is used for ATP monitoring to measure cell viability and bacterial count. It is also used for reporter gene detection. It can be used with the small animal in vivo imaging system for in vivo fluorescence detection after labeling the LUC gene.
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