Product Introduction:

Mitochondrial membrane potential detection kit (JC-1) is a fluorescence probe using JC-1 to quickly and sensitively detect changes in membrane potential of cells, tissues or purified mitochondria, which can be used for early apoptosis detection. JC-1 is an ideal fluorescent probe for detecting mitochondrial membrane potential △Ψm. Can detect cell, tissue or purified mitochondrial membrane potential. When the membrane potential of mitochondria is high, JC-1 accumulates in the matrix of mitochondria and forms a polymer, which can produce red fluorescence. When the mitochondrial membrane potential is low, JC-1 cannot accumulate in the matrix of mitochondria, and at this time JC-1 is a monomer and can produce green fluorescence. Thus, it is very convenient to detect changes in mitochondrial membrane potential through the transformation of fluorescence color. The proportion of mitochondrial depolarization is often measured by the relative ratio of red-green fluorescence.

The decrease of mitochondrial membrane potential is a landmark event in the early stage of apoptosis. The decrease of cell membrane potential can be easily detected by the transformation of JC-1 from red fluorescence to green fluorescence, and the transformation of JC-1 from red fluorescence to green fluorescence can also be used as an early detection index of apoptosis. The maximum excitation wavelength and emission wavelength of JC-1 monomer are 515nm and 529nm respectively. The maximum excitation wavelength of JC-1 polymer is 585nm and the maximum emission wavelength is 590nm. For actual observation, use the conventional Settings for observing red and green fluorescence.

This kit provides CCCP as a positive control for induced mitochondrial membrane potential decline. For samples in six-well plates, the kit can detect a total of 100 samples; For samples in 12 Wells, this kit can test a total of 200 samples.

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