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Storage:Store at -20℃，avoid light，1 year
Product Introduction:
Mitochondrial membrane potential detection kit (JC-1) is a fluorescence probe using JC-1 to quickly and sensitively detect changes in membrane potential of cells, tissues or purified mitochondria, which can be used for early apoptosis detection. JC-1 is an ideal fluorescent probe for detecting mitochondrial membrane potential △Ψm. Can detect cell, tissue or purified mitochondrial membrane potential. When the membrane potential of mitochondria is high, JC-1 accumulates in the matrix of mitochondria and forms a polymer, which can produce red fluorescence. When the mitochondrial membrane potential is low, JC-1 cannot accumulate in the matrix of mitochondria, and at this time JC-1 is a monomer and can produce green fluorescence. Thus, it is very convenient to detect changes in mitochondrial membrane potential through the transformation of fluorescence color. The proportion of mitochondrial depolarization is often measured by the relative ratio of red-green fluorescence.
The decrease of mitochondrial membrane potential is a landmark event in the early stage of apoptosis. The decrease of cell membrane potential can be easily detected by the transformation of JC-1 from red fluorescence to green fluorescence, and the transformation of JC-1 from red fluorescence to green fluorescence can also be used as an early detection index of apoptosis. The maximum excitation wavelength and emission wavelength of JC-1 monomer are 515nm and 529nm respectively. The maximum excitation wavelength of JC-1 polymer is 585nm and the maximum emission wavelength is 590nm. For actual observation, use the conventional Settings for observing red and green fluorescence.
This kit provides CCCP as a positive control for induced mitochondrial membrane potential decline. For samples in six-well plates, the kit can detect a total of 100 samples; For samples in 12 Wells, this kit can test a total of 200 samples.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
染色荧光观察发现几乎整个细胞都染上了，有什么解决办法2022-08-09 18:25:36
Administrator您好，可以降低显微镜光源强度或者用激光共聚焦显微镜观察，荧光背景值要更低一些；还有就是染色工作液孵育后可以对样本多做一下清洗，未装载进入细胞的游离的荧光探针要清洗掉；此外就是可以根据细胞密度调整降低下工作浓度，成像拍摄使用分辨率更高的倍镜检测等。
说明书中关于纯化线粒体的方法，用荧光酶标仪 激发波长485nm，发射波长590nm检测出的数值是何意义？2022-07-26 17:14:23
Administrator您好，荧光酶标仪检测也是根据激发发射波长检测对应的荧光值读数，最后实验结果体现在数据上的方法，纯化的线粒体一般建议用这种方法，因为显微镜或流式等检测手段更多针对于细胞样本，线粒体细胞器结构是要更小的，纤维镜拍摄比较难以观察。