Product introduction

dnase for Campylobacter

preparation method

42.0g of this product was weighed and dissolved in 1000ml distilled water by slow heating. The product was divided into triangular bottles and autoclaved at 121℃ for 15 minutes, and cooled to about 50℃. For every 100ml of medium, 1ml of 0.5% methyl green water solution filtered to remove bacteria was added, mixed, and poured into the plate.

composition: g/L

DNA: 2.0

Phytopeptone: 5.0

Pancreatic casein peptone: 15.0

Sodium chloride: 5.0

Agar: 15.0

pH: 7.3±0.1, 25℃

Precautions

Methyl green preparation (MG) : 0.5% methyl green aqueous solution was prepared. The same volume of chloroform was extracted 5-7 times until the chloroform layer was colorless. 1ml of staining solution was added to each 100ml of sterilized dissolved medium, and the final concentration of methyl green was 0.005%. Methyl green should be prepared by yourself.

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