SSC,20× (pH 5.3)

Product Description:

For Southern blot DNA electrophoresis.

Component concentration

NaCl 3M

Sodium citrate 0.3M

HCl regulates pH to 5.3

saline sodium citrate (SSC) buffer solution is the most standard imprinting and hybridization treatment solution in molecular biology, designed for various hybridization experiments to achieve denaturation and cleaning purposes. The main components are sodium chloride and sodium citrate. Sodium citrate in SSC buffer acts as a buffer, and salt ions (Na) neutralize the negative charge on the nucleic acid main chain to make it neutral, so that the binding of probe and target sequence is easier. Also used for SDS-PAGE electrophoresis separation gel preparation. For nucleic acid hybridization, different concentrations of different effects: commonly used 2×, and 0.5×2×SSC: high-salt wash film, wash away part of the non-specific binding probe. 0.5×SSC: Low salt washing membrane, increase the tightness of nucleic acid chain, so that the repulsion between RNA/DNA increases.

This product is 20× concentrated liquid, which can be diluted directly with deionized water according to experimental requirements.

Note:

1. Pay attention to avoid microbial contamination of the solution during use.

2. Store at room temperature, if the solution is kept at a low temperature, it may precipitate and can be shaken or heated to dissolve.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.