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Your Position: Home > Cell Biology > Cell Culture > Other Auxiliary Reagents > DMSO(Dimethyl sulfoxide)
DMSO(Dimethyl sulfoxide)

CAS:67-68-5

Appearance:Colorless liquid

Storage:RT.

Purity:>99.5%

  D8371Manual
DMSO(Dimethyl sulfoxide)
Cat.No：
D8371
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
D8371-50ml Inquiry Inquiry Inquiry Inquiry
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Product Information Related Products Related Paper COA Technical Q&A

The kit provides an effective means for detecting bacterial activity and provides a mixture of PMA dye and qPCR based on SYBR Green dye. The optimal amount of dye and the number of samples that can be treated may vary depending on the type of sample. PMA is a DNA-binding dye with high affinity, especially with double-stranded DNA. The dye itself has weak fluorescence, but it can emit brighter fluorescence when it binds to nucleic acid. PMA is impermeable to the cell membrane, so it can selectively modify the DNA of dead cells with damaged membrane. After the photolysis of PMA modified DNA by blue light (~ 464 nm), the photoreactive azide group on PMA is transformed into highly reactive azene radical, which reacts with any hydrocarbon part near the DNA binding site to form a stable covalent nitrogen-carbon bond, resulting in permanent DNA modification. This modification process makes DNA insoluble and is lost along with cell fragments in the later stage of genomic DNA extraction. The residual unbound PMA in the solution reacts with water molecules under strong light to decompose into hydroxylamine compounds with no cross-linking activity, so that they can no longer covalently bind DNA. Based on this characteristic of PMA, the kit combines PMA with qPCR technology to form a new detection method (PMA-qPCR), which is used for screening live bacteria. At present, this method has been verified in a variety of bacterial strains as well as yeast, fungi, viruses and parasites. The treatment of complex samples, such as feces or soil, may need to optimize sample dilution, dye concentration and light treatment time. The treatment of diluted samples, such as water testing, may require filtration or concentration before dye treatment.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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