Storage:RT
Product Introduction:
For selective isolation and culture of Fusobacterium.
Preparation Method:
Weigh 53.2g of this product in 1L distilled or deionized water, heat and boil until completely dissolved, then pack, autoclave at 121℃ for 15 minutes or 115℃ for 30 minutes, shake well after sterilization to prevent Agar from settling in the bottom of the vessel and coagulating, set aside. Cold to 55℃, aseptic operation, every 100ml along the bottle wall preheated 35℃ defibrillated sheep blood 5ml; Sterile vancomycin 0.5mg; Oxamycin 0.3mg; 10mg neomycin, mix well, pour plate.
Composition: g/L
Casein peptone: 10.0
Soy peptone: 3.0
Tissue digestives: 10.0
Glucose: 1.0
Yeast Infusion powder: 2.0
Sodium chloride: 5.0
Sodium bisulfite: 0.1
Heme chloride: 2.5mg
Disodium hydrogen phosphate: 5.0
Potassium dihydrogen phosphate: 1.0
Magnesium sulfate: 0.1
TWEEN 80:1.0 ml
Dithiothreitol: 0.01
Agar: 15.0
pH: 7.4±0.2, 25℃
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.