Storage:RT,avoid light
Product Introduction:
YNB and biotin were added to induce expression in Pichia pastoris, and 1% glycerol or 0.5% methanol was added during induction.
Preparation Method:
Weigh 44.1g of this product, boil and dissolve it in 800ml deionized water, autoclaving at 121℃ for 15 minutes, cooling and aseptic operation, add 100ml of 13.4%YNB solution and 2ml of 0.02% biotin solution. Before induction, BMMY medium was prepared by adding 100ml 5% methanol aqueous solution filtered for sterilization, and BMGY medium was prepared by adding 100ml sterile 10% glycerol aqueous solution.
Composition: g/L
Peptone: 20.0
Yeast Infusion powder: 10.0
pH6.0 Phosphate buffer: 100mmol
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