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My CartApplication：ELISA
Reactivity：Human
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：8 pg/ml
Storage：2-8℃，6 months
Gene ID：2247
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| Name | Human FGF basic ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | FGF basic |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Human |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 15.62-1000 pg/ml |
| Sensitivity | 8 pg/ml |
| Specificity | Do not react with Human FGF-9， Human FGF-10， Human FGF-12， Human FGF-16， Human FGF-17， Human FGF-18， Human FGF-19， Human FGF-20， Human FGF-21， Mouse FGF-6， Mouse FGF-8b， Mouse FGF-8c， Mouse FGF-9， Mouse FGF-10， Mouse FGF-23 etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | P09038 |
| Gene ID | 2247 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人FGF basic单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的人FGF basic会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗人FGF basic抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的人FGF basic发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的人FGF basic，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的人FGF basic浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中人FGF basic的浓度。 |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Polyvinyl-alcohol,chitosan and graphene-oxide composed conductive hydrogel for electrically controlled fluorescein sodium transdermal release
Click to check >>Author：Shuting Xiong, Sheng Ye, Panxianzhi Ni, Meng Zhong, Jing Shan, Tun Yuan, Jie Liang, Yujiang Fan, Xingdong Zhang
IF：11.2000
Publish_to：CARBOHYDRATE POLYMERS
PMID：37567713
您好，请问该产品的标准曲线只能够使用线性回归方程拟合吗？对R2有要求吗？可以使用四参数方程拟合曲线吗？谢谢 2023-03-06 11:32:14
Administrator您好，线性拟合或者四参数方程拟合都是可以的；R2越接近1拟合效果越好；建议选择拟合效果更好的来绘制标曲。