Human EGFR ELISA Kit
Cat.No：SEKH-0154 Solarbio
Application： ELISA
Reactivity：Human
Sensitivity：7.8 pg/ml
Storage：2-8℃，6 months
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PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
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Hippo
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Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Product Information
| Name | Human EGFR ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | EGFR |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Human |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 15.62-1000 pg/ml |
| Sensitivity | 7.8 pg/ml |
| Specificity | Do not react with Human Amphiregulin， Human Betacellulin， Human EGF， Human Epiregulin， Human ErbB2， Human ErbB3， Human ErbB4， Human HB-EGF， Human TGF-α， Human TGF-β1， Human TGF-β2， Human TGF-β3， Mouse EGF etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | P00533 |
| Gene ID | 1956 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人EGFR/HER1/ErbB1单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的人EGFR/HER1/ErbB1会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗人EGFR/HER1/ErbB1抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的人EGFR/HER1/ErbB1发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的人EGFR/HER1/ErbB1，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的人EGFR/HER1/ErbB1浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中人EGFR/HER1/ErbB1的浓度。 |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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