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My CartReactivity：Mouse
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：4pg/mL
Storage：2-8℃，6 months
Gene ID：16176
Qty：
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| Name | Mouse IL-1β ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | IL-1β |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Mouse |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Sensitivity | 4pg/mL |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Swiss Prot | P10749 |
| Gene ID | 16176 |
| Detection Principle | Solarbio ELISA kit uses enzyme-linked immunosorbent assay based on double antibody sandwich method. The anti-mouse IL-1β monoclonal antibody was coated on the enzyme-labeled plate. The mice IL-1β in the gradient diluted standard and the pre-diluted sample were added respectively, and the IL-1β in the standard and the sample was fully bound to the coated antibody on the enzyme-labeled plate. After washing the plate, biotinylated anti-mouse IL-1β antibody was added, and the antibody would bind specifically to the mouse IL-1β in the standard and sample coated with the antibody. After washing the plate, horseradish peroxidase (HRP) labeled streptavidin was added, and the biotin and streptavidin had high non-covalent bonding. After washing the plate, TMB was added. If there were different concentrations of mouse IL-1β in the reaction hole, HRP could change colorless TMB into blue substance of different shades (positive correlation), and the reaction hole would turn yellow after adding the termination solution. Finally, the absorbance (OD) of the reaction hole sample was measured at λmax=450 nm (OD=450 nm). The concentration of mouse IL-1β in the sample was proportional to OD, and the concentration of mouse IL-1β in the sample could be calculated by drawing a standard curve and four-parameter fitting software. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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