Product Introduction:

The GUS (β-Galactosidase) gene is a hydrolase encoding β-glucosidase present within the genomes of some bacteria such as E. coli, and the gene is often used as a fusion marker in plant transgenic analyses and regulatory studies.The GUS receptor gene system has expression of the E. coli GUS enzyme is stable and has low activity within the plant, and the vast majority of plants have no The background activity of glucosidase is detected, and the reaction products can be detected by various methods using β-glucosinolates as substrates.GUS stain is the abbreviation of β-Galactosidase Reporter Gene Staining Kit.The staining principle of GUS stain is that under suitable reaction conditions, β-glucosidase will be detected by the reaction products of β-glucosinolates. Under suitable reaction conditions, β-glucosidase (GUS) can hydrolyze X-Gluc into blue substance, which is insoluble in the indigo substance in the nuclear tissue of the transgenic cells, and the site or locus with GUS activity shows blue color or blue spots, which can be observed with the naked eye or microscope.

GUS Stain can be used for biochemical activity analysis, immunoassay and histochemical staining of tissues and cells, mostly used for GUS gene expression analysis of transgenic plants. This reagent is intended for use in scientific research only and is not intended for clinical diagnosis or other uses.

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