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Your Position: Home > Cell Biology > Hypoxanthine
Hypoxanthine

CAS:68-94-0

Appearance:White to off-white powder

Storage:RT.

Purity:Purity≥99%

Hypoxanthine
Cat.No：
H8010
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
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Product Information Related Products Related Paper COA Technical Q&A

Application:

Biochemical reagents, preparation of biological media, such as the preparation of HAT selective media.

Preparation of HAT medium (for reference only) :

100×A (aminopterin) storage solution: weigh 1.76mg aminopterin dissolved in 90ml ultrapure water, add 0.5ml of 1mol/L NaOH drop to help dissolve, after completely dissolved, add 0.5ml of 1mol/L HCL to neutralize, and then add ultrapure water to 100ml. The bacteria were removed by 0.22um membrane filtration, and small amounts were divided into aliquots and stored at -20 ℃.

100×HT storage solution: Weigh 136.1mg hypoxanthine and 38.8mg thymidine, add ultrapure water to 100ml, place in 45-50℃ water bath to dissolve completely, filter to remove bacteria through 0.22μm membrane, aliquots in small amount, store at -20℃, dissolve in 37℃ water bath before clinical use.

HT medium: 82ml basal medium was supplemented with 1ml HT (100×), 1ml SP (100×), and 15ml inactivated calf serum.

HAT medium: 99ml HT medium was added to 1ml A (100×).

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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