Aggregated single-walled carbon nanotubes attenuate the behavioural and neurochemical effects of methamphetamine in mice
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Author：
Xue Xue Jing-Yu Yang Yi He Li-Rong Wang Ping Liu L
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Nature Nanotechnology 11, 613-620 (2016)
STING is a cell-intrinsic metabolic checkpoint restricting aerobic glycolysis by targeting HK2
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NATURE CELL BIOLOGY
Effects of oxidative stress on hepatic encephalopathy pathogenesis in mice
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Bai Yunhu, Li Kenan, Li Xiaodong, Chen Xiyu, Zheng Jie, Wu Feifei, Chen Jinghao, Li Ze, Zhang Shuai, Wu Kun, Chen Yong, Wang Yayun, Yang Yanling
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Nature Communications
Prolonged Fluoride Exposure Induces Spatial-memory Deficit and Hippocampal Dysfunction by Inhibiting Small Heat Shock Protein 22 in Mice
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Jinge Xin, Bin Zhu, Hesong Wang, Yong Zhang, Ning Sun, Xi Cao, Liqin Zheng, Yanxi Zhou, Jing Fang, Bo Jing, Kangcheng Pan, Yan Zeng, Dong Zeng, Fali Li, Yang Xia, Peng Xu, Xueqin Ni
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JOURNAL OF HAZARDOUS MATERIALS
Iron oxide nanoparticles augment the intercellular mitochondrial transfer?mediated therapy
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Targeted Mitochondrial Fluorescence Imaging-Guided Tumor Antimetabolic Therapy with the Imprinted Polymer Nanomedicine Capable of Specifically Recognizing Dihydrofolate Reductase
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Copper exerts cytotoxicity through inhibition of iron-sulfur cluster biogenesis on ISCA1/ISCA2/ISCU assembly proteins
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Jing Du, Zhaoyang Huang, Yanchun Li, Xueying Ren, Chaoting Zhou, Ruolan Liu, Ping Zhang, Guojie Lei, Jianxin Lyu, Jianghui Li, Guoqiang Tan
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FREE RADICAL BIOLOGY AND MEDICINE
Asperuloside,the bioactive compound in edible Eucommia ulmoides male flower,delays muscle aging by daf-16 mediated improving mitochondrial dysfunction
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Qi Chen, Qinjun Zhang, Amel Thanina Amrouche, Weisu Huang, Baiyi Lu
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Food & Function
Low-dose of caffeine alleviates high altitude pulmonary edema via regulating mitochondrial quality control process in AT1 cells
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Author：
Liuyang Tian, Zhilong Jia, Yan Yan, Qian Jia, Wenjie Shi, Saijia Cui, Huining Chen, Yang Han, Xiaojing Zhao, Kunlun He
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Frontiers in Pharmacology
ROS-responsive exogenous functional mitochondria can rescue neural cells post-ischemic stroke
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Author：
Yanjiao Li, Yachao Wang, Weiqi Yang, Zhen Wu, Daiping Ma, Jianxiu Sun, Huixian Tao, Qinlian Ye, Jingnan Liu, Zhaoxia Ma, Lihua Qiu, Weiping Li, Liyan Li, Min Hu
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Frontiers in Cell and Developmental Biology
Celastrol inhibits microglial pyroptosis and attenuates inflammatory reaction in acute spinal cord injury rats
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Author：
WangyingDaiXingguoWangHonglinTengChiLiBinWangJingW
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International Immunopharmacology
COPS6 promotes tumor progression and reduces CD8+ T cell infiltration by repressing IL-6 production to facilitate tumor immune evasion in breast cancer
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Author：
Aiyun Li, Yichao Zhang, Jin Wang, Yan Zhang, Wanzhen Su, Feng Gao, Xiangying Jiao
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JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
Rapamycin protects against paraquat-induced pulmonary fibrosis: Activation of Nrf2 signaling pathway
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Author：
Yiheng Xu,Wenlin Tai,Xiaoyuan Qu,Wenjuan Wu,ZhenKu
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Biochemical and Biophysical Research Communications
WSSV exploits AMPK to activate mTORC2 signaling for proliferation by enhancing aerobic glycolysis
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Author：
Fei Wang, Qian Ma, Xinglu Dong, Tinghuan Wang, Chao Ma
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JOURNAL OF INVESTIGATIVE SURGERY
English
中文
![/></p><p><b> Operation steps: </b></p>A. Tissue homogenate: Weigh 100~200 mg of fresh tissue such as liver, brain, myocardium, etc., rinse with PBS or normal saline, wash off blood water, blot dry with filter paper, cut into pieces with scissors and put them into a small-capacity glass homogenizer. Lysis Buffer with 1.0 mL ice was added and the lysis was grinded up and down for 20 times in a 0℃ ice bath. b. Cultured cell homogenate: digested cells, washed with PBS, centrifuged at 800g for 5-10 min, collected cells and counted them. The Lysis Buffer of 5×10<sup>7</sup> cells was added to 1.0 mL of suspended cells pre-cooled by ice for each extraction. The cell suspension was transferred to a small-capacity glass homogenizer and ground in a 0℃ ice bath for 30~40 times. </p><p>2. Transfer the tissue or cell homogenate to a centrifuge tube and centrifuge at 1000g at 4℃ for 5 min. </p><p>3. Remove the supernatant and transfer it to a new centrifuge tube. Centrifuge again at 1000g at 4℃ for 5 min. </p><p>4. Remove the supernatant and transfer it to a new centrifuge tube, centrifuge at 4℃ at 12,000 g for 10 min. The supernatant after centrifugation contains cytoplasmic components from which cytoplasmic proteins can be extracted. The supernatant is transferred to a new centrifuge tube, and the mitochondria are deposited on the bottom of the tube. </p><p>5. 0.5mL Wash Buffer was added to the mitochondrial precipitation to suspend the mitochondrial precipitation, and centrifuged at 1000 g at 4℃ for 5 min. </p><p>6. Take the supernatant and transfer it to a new centrifuge tube, centrifuge at 4℃ at 12,000 g for 10 min. After the supernatant is discarded, high purity mitochondria are deposited on the bottom of the tube. </p><p>7. Re-suspend mitochondrial precipitation with 50-100 μL Store Buffer or appropriate reaction buffer for immediate use or -70℃ preservation. </p><p><b> Note: </b></p><p>1. In order to obtain complete mitochondria, it is necessary to: first, the whole process of low temperature operation; Second, fast; Third, breaking up cells without destroying suborganelles is the most critical step in making mitochondria. Compared with tissue blocks, cultured cells, especially adherent cells, are more difficult to break the wall when homogenized by glass homogenizer, so small capacity glass homogenizer and tight gap pestle should be used to grind the cultured cells up and down. </p><p>2. Calculate the correct centrifugal speed with centrifugal force g, and different centrifuges can accurately calculate the centrifugal speed accordingly. 3. Western Blot and 2D-gel electrophoresis were performed, and the sample buffer could be directly added to split mitochondria. </p><p> Rotational speed and centrifugal force conversion: </p><p>G = 1.11×(10-5)×R×[rpm]<sup>2</sup>G is the centrifugal force, generally expressed as a multiple of g (acceleration of gravity); [rpm]<sup>2</sup> : the square of the speed; R is the radius in centimeters. </p><p><b>Note：<span style=](/images/upload/image/20160615/20160615160117_27539.png)
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分离到的线粒体是否还具有活性，如何对其活性进行简单评估？2023-07-17 11:54:55
Administrator您好，可以使用G1573线粒体活性染色试剂盒检测纯化的线粒体活性
您好，提取线粒体之后，该如何确定产物为线粒体，以及提取线粒体的完整性2022-09-29 21:25:33
Administrator有健那绿这种活体染色剂，专一用于线粒体的染色，染色原理是与线粒体中细胞色素C氧化酶结合从而出现蓝绿色。
样本采集有要求吗 是否可以先液氮冻存运输回实验室在研磨？2022-07-22 21:49:02
Administrator您好，冻存样本相比新鲜样本提取效率更低以及线粒体结构完整性也会有影响，还要看后面得到的线粒体是用于什么指标检测的，如果涉及到酶活检测、膜电位检测等活性相关实验都不建议使用冻存样本来做了。