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MTT Cell Proliferation and Cytotoxicity Assay Kit

Storage: -20℃, avoid light, valid for 1 year.

MTT Cell Proliferation and Cytotoxicity Assay Kit
Cat.No:
M1020
Brand:
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
M1020-500T Inquiry Inquiry Inquiry Inquiry
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 /></p><p> Product Introduction: </p><p>MTT can be reduced by some dehydrogenases in the mitochondria to form the crystalline dark purple product formazan, which can be completely dissolved in the presence of a specific solvent, and then the absorbance near the wavelength of 490nm can be measured by an enzymoscope. The more cells proliferate and the faster, the higher the absorbance; The greater the cytotoxicity, the lower the absorbance. </p><p> Instructions for use: </p><p>1. Cells of logarithmic phase were collected and the concentration of cell suspension was adjusted and divided into 96-well plates with 180μL per well and 3000-10000 cells/well. </p><p>2. The cells were cultured at 37℃ and 5%CO2 for 6-24 hours. </p><p>3. Add the appropriate concentration of the tested compound and continue to culture for an appropriate time. </p><p>4. Carefully absorb the supernatant, add 90μL fresh culture solution, then add 10μL MTT solution, and continue to culture for 4 h. </p>5. Then absorb the supernatant, add 110μL Formazan solution to each well, and oscillate at a low speed for 10 min on a shaking table to fully dissolve the crystal. The light absorption values of each hole were measured at 490 nm by enzyme-linked immunoassay. </p><p>6. Zero holes (medium, MTT, Formazan solution) and control holes (cells, drug dissolving medium of the same concentration, culture medium, MTT, Formazan solution) were set up at the same time, and 3 multiple holes were set up in each group. </p><p> Note: </p><p>1. Due to the use of 96-well plates for detection, if the cell culture time is long, we must pay attention to the problem of evaporation. On the one hand, since a circle around the 96-well plate is the easiest to evaporate, the method of abandoning the circle around the plate can be adopted to add PBS, water or culture solution; On the other hand, the 96-well plate can be placed close to the water source in the incubator to ease evaporation. </p><p>2. The MTT solution is yellow and should be kept away from light. Long-term light will cause failure. Do not use when the color turns grayish green. Formazan solution can be incubated in a water bath at 37 ° C to promote dissolution when frozen or precipitated, and must be used after all dissolving and mixing. </p><p>3. For your safety and health, please wear a lab coat and disposable gloves. </p><p><b>Note:<span style=Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark:
These protocols are for reference only. Solarbio does not independently validate these methods.

Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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