Matrigel is a mixture of extracellular matrix containing laminin and a variety of growth factors for cell culture.

The basement membrane matrix was extracted from EHS mouse tumors rich in extracellular matrix proteins, and its main components included laminin, type IV collagen, nestin, heparin glycoprotein, growth factors and matrix metalloproteinases. At room temperature, the substrate gel polymerizes to form a biologically active three-dimensional matrix, which simulates the structure, composition, physical properties and functions of the basement membrane of cells in vivo, which is conducive to cell culture and differentiation in vitro, and can be used for the study of cell morphology, biochemical function, migration, infection and gene expression.

Use:

Cells can be directly coated without dilution when cultured, and the coating thickness can be 0.5 mm(thin layer) or 1.0 mm(thick layer). Matrigel can also be diluted in serum-free medium and used on the surface of coated culture vessels to determine the corresponding Matrigel concentration according to cell type and application purpose.

Matrigel can be gelatinized at a temperature above 10℃. During the operation, keep Matrigel on ice all the cell culture vessels, pipette heads and dispensing tubes in contact with Matrigel must be pre-cooled before use.

Matrigel substrate will have a color difference (light yellow to deep red), due to phenol red and bicarbonate and CO2 caused by the effect, and 5% CO2 equilibrium will reduce the color difference. After freezing and thawing, gently shake the reagent bottle to disperse Matrigel evenly. All operations must be carried out in a sterile environment, the reagent bottle cap can be wiped with 70% ethanol, and natural drying. A pre-cooled pipette should be used to ensure that Matrigel is homogenized.

The Matrigel substrate can be dissolved overnight on ice in a refrigerator at 4℃. Matrigel can be returned to liquid form at 4℃ for 24-48h.

Application:

The three-dimensional culture matrix formed by the substrate glue can promote the adhesion and differentiation of epithelial cells, hepatocytes, Sertoli cells, melanoma cells, vascular endothelial cells, thyroid cells and hair follicle cells. At the same time, the basal glue can also affect the protein expression of mammary epithelial cells, support the neovulation of peripheral nerves and the differentiation of bovine tubal epithelial cells. Different formulations of base adhesive can meet different experimental requirements.

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