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Storage:RT
Product Introduction:
Used for bifidobacterium growth culture. One 2.5mg solution of heme chloride and one 1mg solution of vitamin K1 were added to every 100ml medium.
Preparation Method:
Weigh 18.04g of this product, heat it and dissolve it in 500ml distilled water, autoclave it at 121℃ for 15 minutes, cool it to about 50℃, add 0.5ml vitamin K1 solution filtered for sterilization and 2.5ml heme chloride solution (5mg/ml), mix well and set aside.
Composition: g/L
Peptone: 20 Glucose: 5.0
Yeast Infusion powder 10.0 Sodium chloride: 0.08
Calcium chloride: 0.008 Magnesium sulfate: 0.008
Potassium hydrogen phosphate: 0.04 sodium bicarbonate: 0.4
Cysteine hydrochloride 0.5 potassium dihydrogen phosphate 0.04pH: 6.0±0.1, 25℃
Precaution:
Preparation of vitamin K1 solution: weigh vitamin K11g, add anhydrous ethanol 99ml, filter and remove bacteria.
Preparation of heme chloride solution: Weigh 0.5g heme chloride and dissolve in 1mol/L sodium hydroxide solution 1ml plus distilled water to 100ml, autoclave at 121℃, and store in refrigerator.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
