Middle Brook 7H10 Agar
Storage:RT
Preparation Method:
Weigh 3.8g of this product, add 1ml glycerin to 180ml distilled water or deionized water, boil and dissolve, autoclave at 121℃ for 10 minutes. When cooled at 50-55℃, add a OADC bacteria-enhancing solution(LA9560) for aseptic operation and set aside.
Composition: g/L
Ammonium sulfate: 0.5
Potassium dihydrogen phosphate: 1.5
Disodium hydrogen phosphate: 1.5
Sodium citrate: 0.4
Magnesium sulfate: 0.025
Calcium chloride: 0.0005
Zinc sulfate: 0.001
Copper sulfate: 0.001
L-glutamate sodium: 0.5
Ferric ammonium citrate: 0.04
Biotin: 0.0005
Agar: 15.0
Malachite green: 0.00025
Vitamin B6:0.001
pH: 6.6±0.2, 25℃
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:
These protocols are for reference only. Solarbio does not independently validate these
methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.