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Product Introduction:
This product uses the principle that the salt ion concentration inside and outside the cell causes the cell membrane to burst to split non-nucleated red blood cells.
This product has been treated aseptically and is mainly used for the separation and purification of histiocytes digested and dispersed by enzymes, the separation and purification of lymphocytes, and the removal of red blood cells in experiments such as the extraction of histiocytic protein and nucleic acid.
Instruction:
1. 1 times the volume of fresh whole blood, add 3 times the volume of red blood cell lysate. For example, add 3ml of red cell lysate to 1ml of fresh whole blood, gently swirl or reverse mix.
2. Place on ice for 15 minutes, during which gently swirl and mix twice, after the red blood cells are cracked, the solution should be clear and transparent.3. Cell collection: Centrifuge at 4℃, 450×g for 10 min to precipitate white blood cells, and carefully absorb and discard the supernatant.
4. Add twice the volume of red blood cell lysate to the white blood cell precipitation, and gently scroll to fully reinsert the white blood cells. If the starting blood is 1ml, add 2ml of red cell lysate.
5.4 ℃, centrifuge at 450×g for 10 min to precipitate white blood cells, carefully and thoroughly absorb the supernatant.
6. Resuspension cells for follow-up experiments; For RNA extraction, it is best to start with a solution prepared with DEPC water at this step.
(Histocellular treatment: Fresh tissue is digested by pancreatic enzyme or collagenase and dispersed into a single cell suspension, and the supernatant is discarded by centrifugation, and the rest are the same as above.)
Note:
This lysate is a sterile product. Please pay attention to aseptic operation when separating cells for cell culture.
For your safety and health, please wear a lab coat and disposable gloves.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
