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My Cart> Dye Reagents > Dye > Fluorescent Dye > DAPI，4’，6-Diamidino-2-phenylindole dihydrochloride(DAPI)Appearance：Yellow Powder
Molecular Weight：350.25
Storage：Store at -20℃，avoid light，3 years
Molecular Formula：C16H15N5·2HCl
CAS：28718-90-3
Qty：
Size：
| Name | DAPI，4’，6-Diamidino-2-phenylindole dihydrochloride(DAPI) |
| CAS | 28718-90-3 |
| Molecular Formula | C16H15N5·2HCl |
| Molecular Weight | 350.25 |
| Purity/Grade | ≥90% |
| Appearance | Yellow Powder |
| Solubility | 1 mg/mL in Water |
| Storage | Store at -20℃，avoid light，3 years |
| Hazard Code | R：36/37/38 S：26-36 |
| Unit | Piece |
| Specification | 10mg |
Product Introduction：
DAPI is a blue fluorescent dye that can penetrate the cell membrane. When combined with double-stranded DNA, it produces fluorescence more than 20 times stronger than DAPI itself. Compared with ethidium bromide (EB), the staining sensitivity for double-stranded DNA is many times higher.DAPI staining is often used to detect apoptosis, which is observed by fluorescence microscopy or flow cytometry after staining. DAPI is also commonly used for general nuclear staining as well as for staining double-stranded DNA in some specific cases.DAPI had a maximum excitation wavelength of 340nm and a maximum emission wavelength of 488nm. The combination of DAPI and double-stranded DNA resulted in a maximum excitation wavelength of 364nm and a maximum emission wavelength of 454nm.
Prepare stock soluiton:it is dissolved in double distilled water to a final concentration of 1mg/ml. This product should not be dissolved directly with buffer solution. It can be stored for 1 year at -20℃. It is recommended to store in separate packaging to avoid repeated freezing and thawing.When used for nuclear staining, the recommended working concentration of DAPI is 0.5-10μg/ml.
Precaution：
1. DAPI can irritate human body to some extent, so please pay attention to proper protection.
2. all fluorescent dyes have the problem of quenching, so it is recommended to complete the detection on the same day after staining.
3. to slow down the fluorescence quenching, anti-fluorescence quenching mounting solution can be used.
4. For your safety and health, please wear a laboratory coat and wear disposable gloves to operate.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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