Product Introduction:

AO belongs to tricyclic aromatic fuel, can label DNA, RNA, belongs to metachromatic fluorescent dye. The dye is membrane-permeable and can penetrate the cell membrane to stain nuclear DNA and RNA. Therefore, AO is often used to detect intracellular DNA and RNA. The binding modes of AO and nucleic acid are as follows: 1. Intercalative binding, AO is embedded between the base pairs of nucleic acid double strands, and this binding mode is mainly the binding of AO and DNA. Its fluorescence emission peak is 530nm, and it is green fluorescence after excitation; 2. Electrostatic attraction: the positively charged AO and the phosphate (negatively charged) of the single-stranded nucleic acid generate electrostatic attraction and binding. This binding mode is mainly the binding of AO and RNA. Its fluorescence emission peak is 640nm, and it shows red fluorescence after excitation. Thus, AO chimeric into double-stranded DNA molecules appears green, and orange-yellow or orange-red fluorescence when bound to DNA single-stranded or RNA.

AO Detection Kit is mainly composed of AO Stain and AO Stain Buffer, which is often used for the detection of apoptosis. After staining, AO could penetrate the normal cell membrane and make the nucleus appear green or yellow-green fluorescence under a fluorescence microscope. However, in apoptotic cells, chromatin condensation or fragmentation can lead to the formation of apoptotic bodies. AO stained them with dense yellow-green fluorescence or yellow-green fragments. The yellow fluorescence of necrotic cells is weakened or even disappeared. AO staining is often combined with EB staining. EB staining only stained dead cells to produce orange fluorescence, which could distinguish normal cells, apoptotic cells and necrotic cells.

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