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CAS:39262-14-1
Purity:HPLC≥98%
Appearance:White to off-white powder
Storage:Store at 2-8℃，2 years.
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HPLC
The analysis of CK was carried out using an Acchrom S6000 HPLC system equipped with Acchrom Unitary C18 HPLC column (4.6 × 250 mm, 5 μm). An aliquot of 40 μL standard CK at different concentrations (1–100 μM) and samples were injected into the column and detected by Ultraviolet (λ = 203 nm), and peak area was used to generate the calibration curve for quantification of CK in samples. The mobile phase was methanol–acetonitrile-water (45:45:10,v/v/v), and the flow rate was 1.0 mL/min.
References：
Liu D, Jiao S, Wei J, Zhang X, Pei Y, Pei Z, Li J, Du Y. Investigation of absorption, metabolism and toxicity of ginsenosides compound K based on human organ chips. Int J Pharm. 2020 Sep 25;587:119669. doi: 10.1016/j.ijpharm.2020.119669. Epub 2020 Jul 20. PMID: 32702454.
LC-MS/MS
The standard curve of CK and absorption samples were detected by LC-MS/MS system equipped with C18 HPLC column (4.6 × 250 mm, 5 μm) and analyzed by Xcalibur 2.2 SP1 application. An aliquot of 20 μL standard CK at different concentrations (0.05–50 μM) and samples were injected into the column and detected by MS for quantification. The mobile phase was methanol–acetonitrilewater (45:45:10, v/v/v), and the flow rate was 200 μL/min. MS detection was set in the range of 28–45 min.
References：
Liu D, Jiao S, Wei J, Zhang X, Pei Y, Pei Z, Li J, Du Y. Investigation of absorption, metabolism and toxicity of ginsenosides compound K based on human organ chips. Int J Pharm. 2020 Sep 25;587:119669. doi: 10.1016/j.ijpharm.2020.119669. Epub 2020 Jul 20. PMID: 32702454.
