Deoxycholic acid sodium salt

Examples of using this product（for reference only）

LC-MS/MS

Analyzed on a UltiMate 3000 UHPLC system coupled with an Orbitrap Q Exactive HF mass spectrometer. A 90-min separation gradient was established with mobile phase A (2% ACN, 0.1% FA) and mobile phase B (0.1% FA in ACN) (0-8 min, 3%-6% B; 8-9 min, 6% B;9-25 min, 6%-12% B; 25-60 min, 12%-24% B; 60-70 min, 24%-35% B; 70-76 min, 35%-90% B; 76-85 min, 90% B; 85-86 min,90%-6% B; 86-90 min, 6% B), where the detection time for the MS was 25-85 min, and therefore also referred to as the 60 min effective gradient. Peptides were ionized at 2 kV. The MS was operated in DIA mode with an m/z scan range of 350 to 1,500 and a resolution of 60,000. The maximum injection time (IT), automatic gain control (AGC) target, and inclusion list were set to 20 ms,1 ×106, and 40 windows, respectively. High energy collisional dissociation (HCD) was employed for precursor ion fragmentation,where the normalized collision energy was 28. The maximum ion injection time of the MS2 was set to automatic and the resolution was 30,000.

References：

Ma C, Li Y, Li J, Song L, Chen L, Zhao N, Li X, Chen N, Long L, Zhao J, Hou X, Ren L, Yuan X. Comprehensive and deep profiling of the plasma proteome with protein corona on zeolite NaY. J Pharm Anal. 2023 May;13(5):503-513. doi: 10.1016/j.jpha.2023.04.002. Epub 2023 Apr 12. PMID: 37305782; PMCID: PMC10257194.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.