Histamine

Examples of using this product（for reference only）

UHPLC-Q-TOF

The analysis of metabolites was carried out using an UHPLC LC 30 for the chromatographic determination. The extract was injected into a C18 analytical column (2.0 mm I.D × 75 mm, 1.6 μm particle size). The mobile phase was 0.1% formic acid solution (solvent A) and acetonitrile (solvent B). The gradient elution programmer was as follows: 0~2 min, 2% B; 2~13 min, 2–90% B; 13~16 min, 100% B; 16~17 min, 100-2% B; 17–20min, 2% B. The flow rate was of 0.3 mL/min, and the column temperature was set at 40 ℃. The injection volume is 3 μL. The peak detection of the extract was used a X500R Q-TOF mass detector. Both primary mass and secondary fragmentation pattern (MS/ MS) spectra were recorded between m/z 50 and m/z 1000 Da.

References：

[1] Li W , Liu Y , Ye Y ,et al.Chemical profiling and metabolic mechanism of Pixian doubanjiang, a famous condiment in Chinese cuisine[J].LWT- Food Science and Technology, 2021(4):111274.DOI:10.1016/j.lwt.2021.111274.

HILIC-UHPLC–QTRAP?/MS2

All NTs were separated on an Acquity UPLC BEH amide column (2.1 mm × 100 mm, 1.7 μm) equipped with an Acquity UPLC BEH Amide VanGuard Pre-column. The mobile phase was A (0.2% formic acid-5 mmol?L-1 ammonium formate and ammonium acetate water solution) and B (0.2% formic acid-1 mmol?L-1 ammonium formate and ammonium acetate water solution). The gradient elution was 0–3.0 min, 85–80% B;3.0–4.0 min, 80–54% B; 4.0–4.5 min, 54–54% B; 4.5–5.0 min, 54–85% B; and 5.0–6.0 min, 85–85% B. The equilibration time was 1 min, and the flow rate of mobile phase was set at 0.4 mL?min?1 with a sample injection volume of 2 μL. The column temperature was set at 30 ℃.

References：

[1]Zhou, Gui-ShengZhang, JingYin, YouTan, Ya-JieTao, Hui-JuanChen, Jia-QianPu, Zong-JinZhu, Zhen-HuaShi, Xu-QinTang, Yu-PingDuan, Jin-Ao.HILIC-UHPLC-QTRAP (R)/MS2 quantification of 15 neurotransmitters of the combination of donepezil and ginkgo ketoester tablet in different biological matrices from dementia mice: Application to study the synergistic effect of the two drugs[J].Microchemical Journal: Devoted to the Application of Microtechniques in all Branches of Science, 2021, 161(1).

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.