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CAS:51-45-6
Purity:HPLC≥98%
Appearance:Light yellow to yellow powder
Storage:Store at 2-8℃，2 years.
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UHPLC-Q-TOF
The analysis of metabolites was carried out using an UHPLC LC 30 for the chromatographic determination. The extract was injected into a C18 analytical column (2.0 mm I.D × 75 mm, 1.6 μm particle size). The mobile phase was 0.1% formic acid solution (solvent A) and acetonitrile (solvent B). The gradient elution programmer was as follows: 0~2 min, 2% B; 2~13 min, 2–90% B; 13~16 min, 100% B; 16~17 min, 100-2% B; 17–20min, 2% B. The flow rate was of 0.3 mL/min, and the column temperature was set at 40 ℃. The injection volume is 3 μL. The peak detection of the extract was used a X500R Q-TOF mass detector. Both primary mass and secondary fragmentation pattern (MS/ MS) spectra were recorded between m/z 50 and m/z 1000 Da.
References：
[1] Li W , Liu Y , Ye Y ,et al.Chemical profiling and metabolic mechanism of Pixian doubanjiang, a famous condiment in Chinese cuisine[J].LWT- Food Science and Technology, 2021(4):111274.DOI:10.1016/j.lwt.2021.111274.
HILIC-UHPLC–QTRAP?/MS2
All NTs were separated on an Acquity UPLC BEH amide column (2.1 mm × 100 mm, 1.7 μm) equipped with an Acquity UPLC BEH Amide VanGuard Pre-column. The mobile phase was A (0.2% formic acid-5 mmol?L-1 ammonium formate and ammonium acetate water solution) and B (0.2% formic acid-1 mmol?L-1 ammonium formate and ammonium acetate water solution). The gradient elution was 0–3.0 min, 85–80% B;3.0–4.0 min, 80–54% B; 4.0–4.5 min, 54–54% B; 4.5–5.0 min, 54–85% B; and 5.0–6.0 min, 85–85% B. The equilibration time was 1 min, and the flow rate of mobile phase was set at 0.4 mL?min?1 with a sample injection volume of 2 μL. The column temperature was set at 30 ℃.
References：
[1]Zhou, Gui-ShengZhang, JingYin, YouTan, Ya-JieTao, Hui-JuanChen, Jia-QianPu, Zong-JinZhu, Zhen-HuaShi, Xu-QinTang, Yu-PingDuan, Jin-Ao.HILIC-UHPLC-QTRAP (R)/MS2 quantification of 15 neurotransmitters of the combination of donepezil and ginkgo ketoester tablet in different biological matrices from dementia mice: Application to study the synergistic effect of the two drugs[J].Microchemical Journal: Devoted to the Application of Microtechniques in all Branches of Science, 2021, 161(1).
